The present paper intends to specify the impact of organizational culture on job satisfaction of the School Teachers of Khairpur city-Pakistan. Job-satisfaction is an essential component of human beings in a civil society. the individual’s assessment within societal environ for achievement of the desired goal and positive enthusiastic condition from the judgment of one’s job or job experience the organizational culture represents the performance of the individuals in the job- market. The actual nature of an employee is tallied with respect to such an organization awhile working job. However, the flexible culture entails the suitable working environ to the employees. Organizational system mostly states the various factors like jargon language; good performance; job environ; company’s laws; job experience; and so forth that assist the employees for improvement of social life and skills in the work, and communicating its rules norms and values and discipline of an organization. Hence, the quantitative strategy employed for data acquisition and running out through regression analysis for such the work. The study revealed that the various factors are linked with job satisfaction within the organization based on the performance and experiences of the individuals in this perspective in a civil society. Further, the raw data is analyzed through (SPSS) software, and other techniques used for analyzing the statistical data Non-parametric tests, ANOVA, reliability, Linear Regression, and correlation Chi-Square test based on the collected raw data from individuals.